A systematic investigation of the bio-toxicity of core-shell magnetic mesoporous silica microspheres using zebrafish model

Gheyath K. Nasrallah, Yu Zhang, Moustafa M. Zagho, Hesham M. Ismail, Areej Abdulkareem Al-Khalaf, Rafael M. Prieto, Kholoud E. Albinali, Ahmed A. Elzatahry, Yonghui Deng

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Abstract

In this work, shearing interface coassembly in biliquid phase systems is employed to synthesize biocompatible core-shell magnetic mesoporous silica microspheres with uniform size of about 600 nm, perpendicular mesopores of 6.0 nm and large pore volume of 0.77 cm3/g. The toxicology assays based on the zebrafish model was conducted to test under a high-throughput manner for the biosafety of Fe3O4@RF@mSiO2 microspheres. The highest no observed toxic effect concentration (NOEC) estimated by the acute toxicity assay for the microspheres was 1.6 mg/mL. The estimated number (measured by ICP-MS) of the penetrated microspheres at this concentration was 2 × 106 per embryo. The results of three different performed toxicity assays show no overall acute toxicity, teratogenicity, or neurotoxicity of the microspheres on zebrafish embryos at any of the tested concentrations (from 0.1 to 1.6 mg/mL) via its multifunctional microstructure. Here, gemcitabine (GEM) as a model of anti-cancer drug was loaded into the mesopores of Fe3O4@RF@mSiO2 microspheres to study their drug release behavior. The microspheres were found to exhibit pH responsive property, which benefits for the GEM release under cancer therapy. Overall, this study offers promising avenue for effective evaluation of magnetic core-shell microspheres for drug delivery and cancer therapy applications.

Original languageEnglish
Pages (from-to)195-201
Number of pages7
JournalMicroporous and Mesoporous Materials
Volume265
DOIs
Publication statusPublished - 15 Jul 2018

Fingerprint

Microspheres
toxicity
Silicon Dioxide
Toxicity
drugs
cancer
embryos
Silica
silicon dioxide
therapy
gemcitabine
toxicology
magnetic cores
inductively coupled plasma mass spectrometry
shearing
Assays
delivery
porosity
microstructure
evaluation

Keywords

  • Acute toxicity
  • Core-shell microspheres
  • Magnetic nanomaterials
  • Neurotoxicity
  • Teratogenicity

Cite this

Nasrallah, Gheyath K. ; Zhang, Yu ; Zagho, Moustafa M. ; Ismail, Hesham M. ; Al-Khalaf, Areej Abdulkareem ; Prieto, Rafael M. ; Albinali, Kholoud E. ; Elzatahry, Ahmed A. ; Deng, Yonghui. / A systematic investigation of the bio-toxicity of core-shell magnetic mesoporous silica microspheres using zebrafish model. In: Microporous and Mesoporous Materials. 2018 ; Vol. 265. pp. 195-201.
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A systematic investigation of the bio-toxicity of core-shell magnetic mesoporous silica microspheres using zebrafish model. / Nasrallah, Gheyath K.; Zhang, Yu; Zagho, Moustafa M.; Ismail, Hesham M.; Al-Khalaf, Areej Abdulkareem; Prieto, Rafael M.; Albinali, Kholoud E.; Elzatahry, Ahmed A.; Deng, Yonghui.

In: Microporous and Mesoporous Materials, Vol. 265, 15.07.2018, p. 195-201.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - A systematic investigation of the bio-toxicity of core-shell magnetic mesoporous silica microspheres using zebrafish model

AU - Nasrallah, Gheyath K.

AU - Zhang, Yu

AU - Zagho, Moustafa M.

AU - Ismail, Hesham M.

AU - Al-Khalaf, Areej Abdulkareem

AU - Prieto, Rafael M.

AU - Albinali, Kholoud E.

AU - Elzatahry, Ahmed A.

AU - Deng, Yonghui

PY - 2018/7/15

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N2 - In this work, shearing interface coassembly in biliquid phase systems is employed to synthesize biocompatible core-shell magnetic mesoporous silica microspheres with uniform size of about 600 nm, perpendicular mesopores of 6.0 nm and large pore volume of 0.77 cm3/g. The toxicology assays based on the zebrafish model was conducted to test under a high-throughput manner for the biosafety of Fe3O4@RF@mSiO2 microspheres. The highest no observed toxic effect concentration (NOEC) estimated by the acute toxicity assay for the microspheres was 1.6 mg/mL. The estimated number (measured by ICP-MS) of the penetrated microspheres at this concentration was 2 × 106 per embryo. The results of three different performed toxicity assays show no overall acute toxicity, teratogenicity, or neurotoxicity of the microspheres on zebrafish embryos at any of the tested concentrations (from 0.1 to 1.6 mg/mL) via its multifunctional microstructure. Here, gemcitabine (GEM) as a model of anti-cancer drug was loaded into the mesopores of Fe3O4@RF@mSiO2 microspheres to study their drug release behavior. The microspheres were found to exhibit pH responsive property, which benefits for the GEM release under cancer therapy. Overall, this study offers promising avenue for effective evaluation of magnetic core-shell microspheres for drug delivery and cancer therapy applications.

AB - In this work, shearing interface coassembly in biliquid phase systems is employed to synthesize biocompatible core-shell magnetic mesoporous silica microspheres with uniform size of about 600 nm, perpendicular mesopores of 6.0 nm and large pore volume of 0.77 cm3/g. The toxicology assays based on the zebrafish model was conducted to test under a high-throughput manner for the biosafety of Fe3O4@RF@mSiO2 microspheres. The highest no observed toxic effect concentration (NOEC) estimated by the acute toxicity assay for the microspheres was 1.6 mg/mL. The estimated number (measured by ICP-MS) of the penetrated microspheres at this concentration was 2 × 106 per embryo. The results of three different performed toxicity assays show no overall acute toxicity, teratogenicity, or neurotoxicity of the microspheres on zebrafish embryos at any of the tested concentrations (from 0.1 to 1.6 mg/mL) via its multifunctional microstructure. Here, gemcitabine (GEM) as a model of anti-cancer drug was loaded into the mesopores of Fe3O4@RF@mSiO2 microspheres to study their drug release behavior. The microspheres were found to exhibit pH responsive property, which benefits for the GEM release under cancer therapy. Overall, this study offers promising avenue for effective evaluation of magnetic core-shell microspheres for drug delivery and cancer therapy applications.

KW - Acute toxicity

KW - Core-shell microspheres

KW - Magnetic nanomaterials

KW - Neurotoxicity

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EP - 201

JO - Microporous and Mesoporous Materials

JF - Microporous and Mesoporous Materials

SN - 1387-1811

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