Aging related changes of retina and optic nerve of uromastyx aegyptia and falco tinnunculus

Hassan I H El-Sayyad, Soad A. Khalifa, Asma S. Al-Gebaly, Ahmed A. El-Mansy

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Aging is a biological phenomenon that involves gradual degradation of the structure and function of the retina and optic nerve. To our knowledge, little is known about the aging-related ocular cell loss in avian (Falco tinnunculus) and reptilian species (Uromastyx aegyptia). A selected 90 animals of pup, middle, and old age U. aegyptia (reptilian) and F. tinnunculus (avian) were used. The retinae and optic nerves were investigated by light and transmission electron microscopy (TEM) and assessments of neurotransmitters, antioxidant enzymes (catalase, superoxide dismustase and glutathione s transferase), caspase-3 and -7, malonadialdhyde, and DNA fragmentation. Light and TEM observations of the senile specimens revealed apparent deterioration of retinal cell layers, especially the pigmented epithelium and photoreceptor outer segments. Their inclusions of melanin were replaced by lipofuscins. Also, vacuolar degeneration and demyelination of the optic nerve axons were detected. Concomitantly, there was a marked increase of oxidative stress involved reduction of neurotransmitters and antioxidant enzymes and an increase of lipid peroxidation, caspase-3 and -7, subG0/G1 apoptosis, and P53. We conclude that aging showed an inverse relationship with the neurotransmitters and antioxidant enzymes and a linear relationship of caspases, malondialdhyde, DNA apoptosis, and P53 markers of cell death. These markers reflected the retinal cytological alterations and lipofuscin accumulation within inner segments.

Original languageEnglish
Pages (from-to)39-50
Number of pages12
JournalACS Chemical Neuroscience
Volume5
Issue number1
DOIs
Publication statusPublished - 15 Jan 2014

Fingerprint

Optic Nerve
Neurotransmitter Agents
Caspase 7
Retina
Lipofuscin
Optics
Antioxidants
Aging of materials
Transmission Electron Microscopy
Caspase 3
Enzymes
Apoptosis
Transmission electron microscopy
Biological Phenomena
Light
Oxidative stress
DNA
Melanins
Demyelinating Diseases
DNA Fragmentation

Keywords

  • Aging
  • Falco tinnunculus
  • optic nerve
  • retina
  • Uromastyx aegyptia

Cite this

El-Sayyad, Hassan I H ; Khalifa, Soad A. ; Al-Gebaly, Asma S. ; El-Mansy, Ahmed A. / Aging related changes of retina and optic nerve of uromastyx aegyptia and falco tinnunculus. In: ACS Chemical Neuroscience. 2014 ; Vol. 5, No. 1. pp. 39-50.
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Aging related changes of retina and optic nerve of uromastyx aegyptia and falco tinnunculus. / El-Sayyad, Hassan I H; Khalifa, Soad A.; Al-Gebaly, Asma S.; El-Mansy, Ahmed A.

In: ACS Chemical Neuroscience, Vol. 5, No. 1, 15.01.2014, p. 39-50.

Research output: Contribution to journalArticle

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