Analysis of Quercetin and Kaempferol in an Alcoholic Extract of Convolvulus pilosellifolius using HPLC

Asma’a Al-Rifai, Ahmad Aqel, Amani Awaad, Zeid A. ALOthman

Research output: Contribution to journalArticleResearchpeer-review

6 Citations (Scopus)

Abstract

A simple, rapid, and sensitive high-performance liquid chromatography (HPLC) method was developed and validated for identification and determination of flavonoids in Convolvulus pilosellifolius. The chromatographic separation was achieved in less than 6 min using C18 column (150 × 4.6 mm, 3 μm) with isocratic mixture of methanol and water containing 0.1 percent v/v formic acid in the ration of 80:20 at 258 nm with a flow rate of 0.4 mL/min. The method was validated in the linear calibration curve ranged between 1 and 300 μg/mL with detection limits of 0.39 and 0.26 μg/mL and quantification limits of 1.20 and 0.79 μg/mL for quercetin and kaempferol, respectively. Good repeatability of the method were achieved at percent relative standard deviation (RSD < 2.18 percent) with respect to inter- and intraday repeatability. Recovery values were found to be in the range of 98.2–100.2 percent, indicating high accuracy of the method. The maximum flavonoid contents were 1.07 and 1.54 percent for quercetin and kaempferol, respectively.

Original languageEnglish
Pages (from-to)1411-1418
Number of pages8
JournalCommunications in Soil Science and Plant Analysis
Volume46
Issue number11
DOIs
Publication statusPublished - 1 Jan 2015

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Convolvulus
kaempferol
alcohol abuse
quercetin
liquid chromatography
high performance liquid chromatography
extracts
repeatability
flavonoids
formic acid
methodology
methanol
detection limit
calibration
analysis
method
water

Keywords

  • Column liquid chromatography
  • Convolvulus pilosellifolius
  • flavonoids
  • plant extract

Cite this

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title = "Analysis of Quercetin and Kaempferol in an Alcoholic Extract of Convolvulus pilosellifolius using HPLC",
abstract = "A simple, rapid, and sensitive high-performance liquid chromatography (HPLC) method was developed and validated for identification and determination of flavonoids in Convolvulus pilosellifolius. The chromatographic separation was achieved in less than 6 min using C18 column (150 × 4.6 mm, 3 μm) with isocratic mixture of methanol and water containing 0.1 percent v/v formic acid in the ration of 80:20 at 258 nm with a flow rate of 0.4 mL/min. The method was validated in the linear calibration curve ranged between 1 and 300 μg/mL with detection limits of 0.39 and 0.26 μg/mL and quantification limits of 1.20 and 0.79 μg/mL for quercetin and kaempferol, respectively. Good repeatability of the method were achieved at percent relative standard deviation (RSD < 2.18 percent) with respect to inter- and intraday repeatability. Recovery values were found to be in the range of 98.2–100.2 percent, indicating high accuracy of the method. The maximum flavonoid contents were 1.07 and 1.54 percent for quercetin and kaempferol, respectively.",
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Analysis of Quercetin and Kaempferol in an Alcoholic Extract of Convolvulus pilosellifolius using HPLC. / Al-Rifai, Asma’a; Aqel, Ahmad; Awaad, Amani; ALOthman, Zeid A.

In: Communications in Soil Science and Plant Analysis, Vol. 46, No. 11, 01.01.2015, p. 1411-1418.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Analysis of Quercetin and Kaempferol in an Alcoholic Extract of Convolvulus pilosellifolius using HPLC

AU - Al-Rifai, Asma’a

AU - Aqel, Ahmad

AU - Awaad, Amani

AU - ALOthman, Zeid A.

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AB - A simple, rapid, and sensitive high-performance liquid chromatography (HPLC) method was developed and validated for identification and determination of flavonoids in Convolvulus pilosellifolius. The chromatographic separation was achieved in less than 6 min using C18 column (150 × 4.6 mm, 3 μm) with isocratic mixture of methanol and water containing 0.1 percent v/v formic acid in the ration of 80:20 at 258 nm with a flow rate of 0.4 mL/min. The method was validated in the linear calibration curve ranged between 1 and 300 μg/mL with detection limits of 0.39 and 0.26 μg/mL and quantification limits of 1.20 and 0.79 μg/mL for quercetin and kaempferol, respectively. Good repeatability of the method were achieved at percent relative standard deviation (RSD < 2.18 percent) with respect to inter- and intraday repeatability. Recovery values were found to be in the range of 98.2–100.2 percent, indicating high accuracy of the method. The maximum flavonoid contents were 1.07 and 1.54 percent for quercetin and kaempferol, respectively.

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