Anti-oxidative and DNA protecting effects of flavonoids-rich Scutellaria lateriflora

Madhukar Lohani, Manuj Ahuja, Manal A. Buabeid, Dean Schwartz, Dennis Shannon, Vishnu Suppiramaniam, Barbara Kemppainen, Muralikrishnan Dhanasekaran

Research output: Contribution to journalArticleResearchpeer-review

9 Citations (Scopus)

Abstract

Scutellaria lateriflora (American skullcap), a native plant of North America, has been used by Americans and Europeans as a nerve tonic for more than 200 years. In vivo studies have shown anxiolytic activity of S. lateriflora in animals and humans. However, the neuroprotective mechanisms of S. lateriflora are not fully understood. Oxidative stress plays a vital role in the neurodegenerative and neuropsychiatric diseases such as anxiety, Alzheimer's disease, depression, and Parkinson's disease. Bioactive compounds present in various medicinal plants neutralize or scavenge toxic free radicals and thus suppress oxidative stress. Therefore, the objective of this study was to investigate the antioxidant effects of S. lateriflora. The antioxidant potential of aqueous or ethanolic extracts of S. lateriflora was determined in mouse brain tissue using various biochemical assays. Protective effects of S. lateriflora against oxidative stress induced DNA fragmentation was determined using plasmid DNA. The ethanolic and aqueous extracts scavenged the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals. The ethanolic extract reduced tert-butyl peroxide-induced reactive oxygen species (ROS) and lipid peroxides in the mouse brain homogenates. Furthermore, the ethanolic extract of S. lateriflora protected hydrogen peroxide-UV induced cleavage of supercoiled plasmid DNA. In conclusion, S. lateriflora exhibited significant antioxidant effects. The current findings posit S. lateriflora as one of the potential experimental herbal drugs that should be screened for its therapeutic potential against various oxidative stress associated mental disorders.

Original languageEnglish
Pages (from-to)1415-1418
Number of pages4
JournalNatural Product Communications
Volume8
Issue number10
Publication statusPublished - 1 Jan 2013

Fingerprint

Scutellaria lateriflora
Scutellaria
Flavonoids
Oxidative Stress
flavonoids
Antioxidants
DNA
Plasmids
oxidative stress
Superhelical DNA
Lipid Peroxides
Poisons
Anti-Anxiety Agents
Brain
DNA Fragmentation
Medicinal Plants
North America
extracts
Mental Disorders
Neurodegenerative Diseases

Keywords

  • Antioxidants
  • Flavonoids
  • Neuroprotection
  • Oxidative stress
  • Scutellaria lateriflora

Cite this

Lohani, M., Ahuja, M., Buabeid, M. A., Schwartz, D., Shannon, D., Suppiramaniam, V., ... Dhanasekaran, M. (2013). Anti-oxidative and DNA protecting effects of flavonoids-rich Scutellaria lateriflora. Natural Product Communications, 8(10), 1415-1418.
Lohani, Madhukar ; Ahuja, Manuj ; Buabeid, Manal A. ; Schwartz, Dean ; Shannon, Dennis ; Suppiramaniam, Vishnu ; Kemppainen, Barbara ; Dhanasekaran, Muralikrishnan. / Anti-oxidative and DNA protecting effects of flavonoids-rich Scutellaria lateriflora. In: Natural Product Communications. 2013 ; Vol. 8, No. 10. pp. 1415-1418.
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Lohani, M, Ahuja, M, Buabeid, MA, Schwartz, D, Shannon, D, Suppiramaniam, V, Kemppainen, B & Dhanasekaran, M 2013, 'Anti-oxidative and DNA protecting effects of flavonoids-rich Scutellaria lateriflora', Natural Product Communications, vol. 8, no. 10, pp. 1415-1418.

Anti-oxidative and DNA protecting effects of flavonoids-rich Scutellaria lateriflora. / Lohani, Madhukar; Ahuja, Manuj; Buabeid, Manal A.; Schwartz, Dean; Shannon, Dennis; Suppiramaniam, Vishnu; Kemppainen, Barbara; Dhanasekaran, Muralikrishnan.

In: Natural Product Communications, Vol. 8, No. 10, 01.01.2013, p. 1415-1418.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Lohani, Madhukar

AU - Ahuja, Manuj

AU - Buabeid, Manal A.

AU - Schwartz, Dean

AU - Shannon, Dennis

AU - Suppiramaniam, Vishnu

AU - Kemppainen, Barbara

AU - Dhanasekaran, Muralikrishnan

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Lohani M, Ahuja M, Buabeid MA, Schwartz D, Shannon D, Suppiramaniam V et al. Anti-oxidative and DNA protecting effects of flavonoids-rich Scutellaria lateriflora. Natural Product Communications. 2013 Jan 1;8(10):1415-1418.