Comparison of ELISA and microscopy for detection of Cryptosporidium oocysts in animals

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Cryptosporidium parasite has gained an attention as an emerging pathogen in the last few decades. It is a zoonotic and is transmitted via the fecal-oral route; this has been implicated as one of the more important opportunistic infections in patients with Acquired immune deficiency syndrome. The results showed the presence of Cryptosporidium oocyst in fecal of sheep (11.1 and 22.2%), goats (15.5 and 10.3%) and camels (18.4 and 22.4%) by using microscopic Ziehl-Neelsen and a serological Enzyme-linked immunosorbent assay, respectively. Since there is no a gold standard technique for detection of Cryptosporidium oocysts; this study aimed to compare the efficacy of microscopic tests in the detection of oocysts in feces with ELISA assay. Results showed that positive samples (22.4 and 22.2%) were detected by ELISA in camels and sheep respectively. Contrary, Ziehl-Neelsen exceeded ELISA in the detection of oocysts in goats samples (15.5 and 10.3%) in goats. Statistical analysis clarified a significant correlation between Ziehl-Neelsen and ELISA for the detection of Cryptosporidium oocysts.

Original languageEnglish
Pages (from-to)341-345
Number of pages5
JournalPakistan Journal of Biological Sciences
Volume18
Issue number7
DOIs
Publication statusPublished - 1 Jan 2015

Fingerprint

Cryptosporidium
oocysts
microscopy
enzyme-linked immunosorbent assay
goats
camels
animals
sheep
acquired immunodeficiency syndrome
gold
mouth
statistical analysis
feces
parasites
sampling
pathogens
assays
infection
testing

Keywords

  • Animals
  • Cryptosporidium
  • Detection
  • ELISA
  • Oocyst
  • Ziehl-Neelsen

Cite this

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title = "Comparison of ELISA and microscopy for detection of Cryptosporidium oocysts in animals",
abstract = "Cryptosporidium parasite has gained an attention as an emerging pathogen in the last few decades. It is a zoonotic and is transmitted via the fecal-oral route; this has been implicated as one of the more important opportunistic infections in patients with Acquired immune deficiency syndrome. The results showed the presence of Cryptosporidium oocyst in fecal of sheep (11.1 and 22.2{\%}), goats (15.5 and 10.3{\%}) and camels (18.4 and 22.4{\%}) by using microscopic Ziehl-Neelsen and a serological Enzyme-linked immunosorbent assay, respectively. Since there is no a gold standard technique for detection of Cryptosporidium oocysts; this study aimed to compare the efficacy of microscopic tests in the detection of oocysts in feces with ELISA assay. Results showed that positive samples (22.4 and 22.2{\%}) were detected by ELISA in camels and sheep respectively. Contrary, Ziehl-Neelsen exceeded ELISA in the detection of oocysts in goats samples (15.5 and 10.3{\%}) in goats. Statistical analysis clarified a significant correlation between Ziehl-Neelsen and ELISA for the detection of Cryptosporidium oocysts.",
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Comparison of ELISA and microscopy for detection of Cryptosporidium oocysts in animals. / AL-Megrin, Wafa A I.

In: Pakistan Journal of Biological Sciences, Vol. 18, No. 7, 01.01.2015, p. 341-345.

Research output: Contribution to journalArticleResearchpeer-review

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