Evaluation of the combination of N-acetylcysteine and or sodium salicylate with ciprofoxacin on bacterial adhesion and biofilm formation on urinary catheters

Ahmed A. Abd El-Aziz, Tarek El-Banna, Fatma I. Sonbol, Amal Abo-Kamar, Doaa W. Seif-Eldin

Research output: Contribution to journalArticleResearchpeer-review

2 Citations (Scopus)

Abstract

Background: Bacteria can adhere to the surface of indwelling urinary devices and forming a biofilm lead to complete blocking to the lumen of the urinary catheter and represent a site of spreading infections. Methods: This study aimed to evaluate the effect of ciprofoxacin (CIP), N-ace-tylcysteine (NAC) and salicylate (SAL) alone and in combinations on both biofilm production and pre-formed mature biofilms on microtiter plates and urinary tract catheter segments. Results: Ciprofoxacin at MIC, 1/2MIC, 1/4MIC and 1/8MIC inhibited biofilm production on microtiter plates by 74.36%, 69.55%, 52.41% and 42.37%, respectively and disrupted the pre-formed biofilms by 53.77%, 47.72%, 42.13% and 34.78%, respectively. Ciprofoxacin also inhibited the biofilm production on catheter segments by 67.35% and 55.22% in presence of MIC and 1/4MIC, respectively and disrupted the pre-formed biofilms by 65.83% in presence of MIC and 51.55% in presence of 1/4MIC. NAC and SAL each alone inhibited biofilm production by up to 71% and 68% on microtiter plates and by up to 62% and 68% on catheter segments, respectively and disrupted pre-formed biofilms by up to 67% and 62% from microtiter plates and by up to 61% and 62% from catheter segments, respectively. Ciprofoxacin/N-acetylcysteine showed higher inhibitory effect on biofilm production and higher disruptive effect on the pre-formed biofilms than ciprofoxa-cin/Salicylate. When salicylate was added to the ciprofoxacin/N-acetylcysteine combination it enhanced the biofilm inhibition to become up to 99% and the disruptive effect became up to 98%. Conclusion: our results revealed the great effect of the tested combinations on inhibition of bacterial adherence and in removal of pre-formed biofilms.

Original languageEnglish
JournalInternational Arabic Journal of Antimicrobial Agents
Volume2
Issue number1
DOIs
Publication statusPublished - 1 Dec 2012

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Sodium Salicylate
Bacterial Adhesion
Urinary Catheters
Acetylcysteine
Biofilms
Salicylates
Catheters
Urinary Tract

Keywords

  • Biofilm
  • Catheter
  • Microtiter plates
  • Non-antibiotics
  • Resistance

Cite this

@article{58fb150b14ea481d92f3a36b35557279,
title = "Evaluation of the combination of N-acetylcysteine and or sodium salicylate with ciprofoxacin on bacterial adhesion and biofilm formation on urinary catheters",
abstract = "Background: Bacteria can adhere to the surface of indwelling urinary devices and forming a biofilm lead to complete blocking to the lumen of the urinary catheter and represent a site of spreading infections. Methods: This study aimed to evaluate the effect of ciprofoxacin (CIP), N-ace-tylcysteine (NAC) and salicylate (SAL) alone and in combinations on both biofilm production and pre-formed mature biofilms on microtiter plates and urinary tract catheter segments. Results: Ciprofoxacin at MIC, 1/2MIC, 1/4MIC and 1/8MIC inhibited biofilm production on microtiter plates by 74.36{\%}, 69.55{\%}, 52.41{\%} and 42.37{\%}, respectively and disrupted the pre-formed biofilms by 53.77{\%}, 47.72{\%}, 42.13{\%} and 34.78{\%}, respectively. Ciprofoxacin also inhibited the biofilm production on catheter segments by 67.35{\%} and 55.22{\%} in presence of MIC and 1/4MIC, respectively and disrupted the pre-formed biofilms by 65.83{\%} in presence of MIC and 51.55{\%} in presence of 1/4MIC. NAC and SAL each alone inhibited biofilm production by up to 71{\%} and 68{\%} on microtiter plates and by up to 62{\%} and 68{\%} on catheter segments, respectively and disrupted pre-formed biofilms by up to 67{\%} and 62{\%} from microtiter plates and by up to 61{\%} and 62{\%} from catheter segments, respectively. Ciprofoxacin/N-acetylcysteine showed higher inhibitory effect on biofilm production and higher disruptive effect on the pre-formed biofilms than ciprofoxa-cin/Salicylate. When salicylate was added to the ciprofoxacin/N-acetylcysteine combination it enhanced the biofilm inhibition to become up to 99{\%} and the disruptive effect became up to 98{\%}. Conclusion: our results revealed the great effect of the tested combinations on inhibition of bacterial adherence and in removal of pre-formed biofilms.",
keywords = "Biofilm, Catheter, Microtiter plates, Non-antibiotics, Resistance",
author = "{Abd El-Aziz}, {Ahmed A.} and Tarek El-Banna and Sonbol, {Fatma I.} and Amal Abo-Kamar and Seif-Eldin, {Doaa W.}",
year = "2012",
month = "12",
day = "1",
doi = "10.3823/708",
language = "English",
volume = "2",
journal = "International Arabic Journal of Antimicrobial Agents",
issn = "2174-9094",
publisher = "Internet Medical Publishing",
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}

Evaluation of the combination of N-acetylcysteine and or sodium salicylate with ciprofoxacin on bacterial adhesion and biofilm formation on urinary catheters. / Abd El-Aziz, Ahmed A.; El-Banna, Tarek; Sonbol, Fatma I.; Abo-Kamar, Amal; Seif-Eldin, Doaa W.

In: International Arabic Journal of Antimicrobial Agents, Vol. 2, No. 1, 01.12.2012.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Evaluation of the combination of N-acetylcysteine and or sodium salicylate with ciprofoxacin on bacterial adhesion and biofilm formation on urinary catheters

AU - Abd El-Aziz, Ahmed A.

AU - El-Banna, Tarek

AU - Sonbol, Fatma I.

AU - Abo-Kamar, Amal

AU - Seif-Eldin, Doaa W.

PY - 2012/12/1

Y1 - 2012/12/1

N2 - Background: Bacteria can adhere to the surface of indwelling urinary devices and forming a biofilm lead to complete blocking to the lumen of the urinary catheter and represent a site of spreading infections. Methods: This study aimed to evaluate the effect of ciprofoxacin (CIP), N-ace-tylcysteine (NAC) and salicylate (SAL) alone and in combinations on both biofilm production and pre-formed mature biofilms on microtiter plates and urinary tract catheter segments. Results: Ciprofoxacin at MIC, 1/2MIC, 1/4MIC and 1/8MIC inhibited biofilm production on microtiter plates by 74.36%, 69.55%, 52.41% and 42.37%, respectively and disrupted the pre-formed biofilms by 53.77%, 47.72%, 42.13% and 34.78%, respectively. Ciprofoxacin also inhibited the biofilm production on catheter segments by 67.35% and 55.22% in presence of MIC and 1/4MIC, respectively and disrupted the pre-formed biofilms by 65.83% in presence of MIC and 51.55% in presence of 1/4MIC. NAC and SAL each alone inhibited biofilm production by up to 71% and 68% on microtiter plates and by up to 62% and 68% on catheter segments, respectively and disrupted pre-formed biofilms by up to 67% and 62% from microtiter plates and by up to 61% and 62% from catheter segments, respectively. Ciprofoxacin/N-acetylcysteine showed higher inhibitory effect on biofilm production and higher disruptive effect on the pre-formed biofilms than ciprofoxa-cin/Salicylate. When salicylate was added to the ciprofoxacin/N-acetylcysteine combination it enhanced the biofilm inhibition to become up to 99% and the disruptive effect became up to 98%. Conclusion: our results revealed the great effect of the tested combinations on inhibition of bacterial adherence and in removal of pre-formed biofilms.

AB - Background: Bacteria can adhere to the surface of indwelling urinary devices and forming a biofilm lead to complete blocking to the lumen of the urinary catheter and represent a site of spreading infections. Methods: This study aimed to evaluate the effect of ciprofoxacin (CIP), N-ace-tylcysteine (NAC) and salicylate (SAL) alone and in combinations on both biofilm production and pre-formed mature biofilms on microtiter plates and urinary tract catheter segments. Results: Ciprofoxacin at MIC, 1/2MIC, 1/4MIC and 1/8MIC inhibited biofilm production on microtiter plates by 74.36%, 69.55%, 52.41% and 42.37%, respectively and disrupted the pre-formed biofilms by 53.77%, 47.72%, 42.13% and 34.78%, respectively. Ciprofoxacin also inhibited the biofilm production on catheter segments by 67.35% and 55.22% in presence of MIC and 1/4MIC, respectively and disrupted the pre-formed biofilms by 65.83% in presence of MIC and 51.55% in presence of 1/4MIC. NAC and SAL each alone inhibited biofilm production by up to 71% and 68% on microtiter plates and by up to 62% and 68% on catheter segments, respectively and disrupted pre-formed biofilms by up to 67% and 62% from microtiter plates and by up to 61% and 62% from catheter segments, respectively. Ciprofoxacin/N-acetylcysteine showed higher inhibitory effect on biofilm production and higher disruptive effect on the pre-formed biofilms than ciprofoxa-cin/Salicylate. When salicylate was added to the ciprofoxacin/N-acetylcysteine combination it enhanced the biofilm inhibition to become up to 99% and the disruptive effect became up to 98%. Conclusion: our results revealed the great effect of the tested combinations on inhibition of bacterial adherence and in removal of pre-formed biofilms.

KW - Biofilm

KW - Catheter

KW - Microtiter plates

KW - Non-antibiotics

KW - Resistance

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U2 - 10.3823/708

DO - 10.3823/708

M3 - Article

VL - 2

JO - International Arabic Journal of Antimicrobial Agents

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SN - 2174-9094

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