Investigation of hemorrhagic fever viruses inside wild populations of ticks

One of the pioneer studies in Saudi Arabia

Rania Ali El Hadi Mohamed, Nahla Mohamed, Fadilah Sfouq Aleanizy, Fulwah Yahya Alqahtani, Areej Al Khalaf, Lamya Ahmed Al-Keridis

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Objective: To screen hemorrhagic fever viruses inside wild populations of ticks collected from Riyadh, Saudi Arabia between January and March 2016. Methods: Ticks were identified depending on their morphological features using classical keys then grouped into pools. Ticks in each pool were processed separately using the sterile pestles and mortars. Viral RNA was extracted using Qiagen RNeasy Mini Kit and Qiagen RNAeasy Columns (Qiagen, Hilden, Germany) according to the instructions of manufacturers. A total number of 1 282 hard ticks were collected, and 582 of them were precisely identified then screened for the presence of arboviruses using quantitative real-time PCR. The four species were screened for six viruses: Rift Valley fever virus (RVFV), Chikungunya virus (CHIKV), Crimean-Congo hemorrhagic fever virus (CCHFV), Alkhurma virus (INKV), Sindbis virus (SINV), and Pan Hanta virus (HANTA). CT value for the negative control (RNA free water) was zero. Negative and positive controls were tested for each test to confirm the specificity of the selected primer pairs. SYBR Green One step RT-PCR Master Mix (KAPA Biosystems, Boston, MA) was tested along with primers. Results: Ticks identification resulted into four species: Hyalomma schulzei, Hyalomma onatoli, Boophilus kdhlsi, and Hyalomm dromedarii. All the ticks' species (except Boophilus kdhlsi) were positive for the following viruses: SINV, RVFV, CHIKV, and CCHFV. While HANTA viruses have been detected in a single species (Hyalomm dromedarii). Conclusions: According to our knowledge this research may be one of the pioneer studies in Kingdom of Saudi Arabia. Incrimination of the above mentioned ticks species as well as their vectorial capacity are highly recommended for investigation in the upcoming researches.

Original languageEnglish
Pages (from-to)299-303
Number of pages5
JournalAsian Pacific Journal of Tropical Disease
Volume7
Issue number5
DOIs
Publication statusPublished - 1 Jan 2017

Fingerprint

Saudi Arabia
Ticks
Fever
Viruses
Crimean-Congo hemorrhagic fever virus
Rift Valley fever virus
Chikungunya virus
Population
Sindbis Virus
Tick-Borne Encephalitis Viruses
Ixodidae
Arboviruses
Viral RNA
Research
Germany
Real-Time Polymerase Chain Reaction
RNA
Polymerase Chain Reaction
Water

Keywords

  • Hemorrhagic fever viruses
  • KSA
  • Wild ticks

Cite this

Mohamed, Rania Ali El Hadi ; Mohamed, Nahla ; Aleanizy, Fadilah Sfouq ; Alqahtani, Fulwah Yahya ; Khalaf, Areej Al ; Al-Keridis, Lamya Ahmed. / Investigation of hemorrhagic fever viruses inside wild populations of ticks : One of the pioneer studies in Saudi Arabia. In: Asian Pacific Journal of Tropical Disease. 2017 ; Vol. 7, No. 5. pp. 299-303.
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abstract = "Objective: To screen hemorrhagic fever viruses inside wild populations of ticks collected from Riyadh, Saudi Arabia between January and March 2016. Methods: Ticks were identified depending on their morphological features using classical keys then grouped into pools. Ticks in each pool were processed separately using the sterile pestles and mortars. Viral RNA was extracted using Qiagen RNeasy Mini Kit and Qiagen RNAeasy Columns (Qiagen, Hilden, Germany) according to the instructions of manufacturers. A total number of 1 282 hard ticks were collected, and 582 of them were precisely identified then screened for the presence of arboviruses using quantitative real-time PCR. The four species were screened for six viruses: Rift Valley fever virus (RVFV), Chikungunya virus (CHIKV), Crimean-Congo hemorrhagic fever virus (CCHFV), Alkhurma virus (INKV), Sindbis virus (SINV), and Pan Hanta virus (HANTA). CT value for the negative control (RNA free water) was zero. Negative and positive controls were tested for each test to confirm the specificity of the selected primer pairs. SYBR Green One step RT-PCR Master Mix (KAPA Biosystems, Boston, MA) was tested along with primers. Results: Ticks identification resulted into four species: Hyalomma schulzei, Hyalomma onatoli, Boophilus kdhlsi, and Hyalomm dromedarii. All the ticks' species (except Boophilus kdhlsi) were positive for the following viruses: SINV, RVFV, CHIKV, and CCHFV. While HANTA viruses have been detected in a single species (Hyalomm dromedarii). Conclusions: According to our knowledge this research may be one of the pioneer studies in Kingdom of Saudi Arabia. Incrimination of the above mentioned ticks species as well as their vectorial capacity are highly recommended for investigation in the upcoming researches.",
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Investigation of hemorrhagic fever viruses inside wild populations of ticks : One of the pioneer studies in Saudi Arabia. / Mohamed, Rania Ali El Hadi; Mohamed, Nahla; Aleanizy, Fadilah Sfouq; Alqahtani, Fulwah Yahya; Khalaf, Areej Al; Al-Keridis, Lamya Ahmed.

In: Asian Pacific Journal of Tropical Disease, Vol. 7, No. 5, 01.01.2017, p. 299-303.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Mohamed, Rania Ali El Hadi

AU - Mohamed, Nahla

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