Longitudinal analysis of the human T cell response during acute hantavirus infection

Therese Lindgren, Clas Ahlm, Nahla Mohamed, Magnus Evander, Hans Gustaf Ljunggren, Niklas K. Björkström

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Abstract

Longitudinal studies of T cell immune responses during viral infections in humans are essential for our understanding of how effector T cell responses develop, clear infection, and provide long-lasting immunity. Here, following an outbreak of a Puumala hantavirus infection in the human population, we longitudinally analyzed the primary CD8 T cell response in infected individuals from the first onset of clinical symptoms until viral clearance. A vigorous CD8 T cell response was observed early following the onset of clinical symptoms, determined by the presence of high numbers of Ki67 +CD38 +HLA-DR + effector CD8 T cells. This response encompassed up to 50% of total blood CD8 T cells, and it subsequently contracted in parallel with a decrease in viral load. Expression levels of perforin and granzyme B were high throughout the initial T cell response and likewise normalized following viral clearance. When monitoring regulatory components, no induction of regulatory CD4 or CD8 T cells was observed in the patients during the infection. However, CD8 as well as CD4 T cells exhibited a distinct expression profile of inhibitory PD-1 and CTLA-4 molecules. The present results provide insight into the development of the T cell response in humans, from the very onset of clinical symptoms following a viral infection to resolution of the disease.

Original languageEnglish
Pages (from-to)10252-10260
Number of pages9
JournalJournal of Virology
Volume85
Issue number19
DOIs
Publication statusPublished - 1 Oct 2011

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Hantavirus Infections
Hantavirus
T-lymphocytes
T-Lymphocytes
infection
signs and symptoms (animals and humans)
Virus Diseases
viral diseases of animals and humans
HLA-DR Antigens
longitudinal studies
viral load
Infection
Viral Load
human population
Disease Outbreaks
Longitudinal Studies
Immunity
immunity

Cite this

Lindgren, T., Ahlm, C., Mohamed, N., Evander, M., Ljunggren, H. G., & Björkström, N. K. (2011). Longitudinal analysis of the human T cell response during acute hantavirus infection. Journal of Virology, 85(19), 10252-10260. https://doi.org/10.1128/JVI.05548-11
Lindgren, Therese ; Ahlm, Clas ; Mohamed, Nahla ; Evander, Magnus ; Ljunggren, Hans Gustaf ; Björkström, Niklas K. / Longitudinal analysis of the human T cell response during acute hantavirus infection. In: Journal of Virology. 2011 ; Vol. 85, No. 19. pp. 10252-10260.
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Lindgren, T, Ahlm, C, Mohamed, N, Evander, M, Ljunggren, HG & Björkström, NK 2011, 'Longitudinal analysis of the human T cell response during acute hantavirus infection', Journal of Virology, vol. 85, no. 19, pp. 10252-10260. https://doi.org/10.1128/JVI.05548-11

Longitudinal analysis of the human T cell response during acute hantavirus infection. / Lindgren, Therese; Ahlm, Clas; Mohamed, Nahla; Evander, Magnus; Ljunggren, Hans Gustaf; Björkström, Niklas K.

In: Journal of Virology, Vol. 85, No. 19, 01.10.2011, p. 10252-10260.

Research output: Contribution to journalArticleResearchpeer-review

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