New Activities for Isolated Compounds from Convolvulus austro-aegyptiacus as Anti-ulcerogenic, Anti-Helicobacter pylori and Their Mimic Synthesis Using Bio-guided Fractionation

Amani S. Awaad, Asmaa A. Al-Rifai, Reham M. El-Meligy, Ahmed M. Alafeefy, Mohamed E. Zain

Research output: Contribution to journalArticleResearchpeer-review

8 Citations (Scopus)

Abstract

Bio-guided fractionation of the total alcoholic extract of Convolvulus austro-aegyptiacus was screened for its anti-ulcerogenic activity, using an absolute-ethanol-induced ulcer model at 500 and 1000mg/kg doses. Two compounds were isolated from the butanol extract of C. austro-aegyptiacus and identified by 1H and 13C nuclear magnetic resonance as scopoletin and scopolin. The isolated compounds (50mg/kg) showed a remarkable anti-ulcerogenic activity because they exhibited control-ulcer protection by 16.7% and 90.8%, respectively. The acute toxicity study showed that the extract is highly safe; the median lethal dose (LD50) was more than 4000mg/kg. Moreover, the obtained results were confirmed by the sub-chronic toxicity because the rats that have been administered 1000mg/kg of the extract for 15 consecutive days showed no alteration in the liver and kidney functions.

Original languageEnglish
Pages (from-to)1311-1316
Number of pages6
JournalPhytotherapy Research
Volume29
Issue number9
DOIs
Publication statusPublished - 1 Sep 2015

Fingerprint

Convolvulus
Helicobacter pylori
Ulcer
Scopoletin
Butanols
Lethal Dose 50
Ethanol
Magnetic Resonance Spectroscopy
Kidney
Liver
tebufenozide

Keywords

  • cytoprotective
  • H. pylori
  • liver and kidney functions
  • peptic ulcer
  • scopoletin
  • scopolin

Cite this

@article{4feee24aabbf4b2caee365d8e21b25ef,
title = "New Activities for Isolated Compounds from Convolvulus austro-aegyptiacus as Anti-ulcerogenic, Anti-Helicobacter pylori and Their Mimic Synthesis Using Bio-guided Fractionation",
abstract = "Bio-guided fractionation of the total alcoholic extract of Convolvulus austro-aegyptiacus was screened for its anti-ulcerogenic activity, using an absolute-ethanol-induced ulcer model at 500 and 1000mg/kg doses. Two compounds were isolated from the butanol extract of C. austro-aegyptiacus and identified by 1H and 13C nuclear magnetic resonance as scopoletin and scopolin. The isolated compounds (50mg/kg) showed a remarkable anti-ulcerogenic activity because they exhibited control-ulcer protection by 16.7{\%} and 90.8{\%}, respectively. The acute toxicity study showed that the extract is highly safe; the median lethal dose (LD50) was more than 4000mg/kg. Moreover, the obtained results were confirmed by the sub-chronic toxicity because the rats that have been administered 1000mg/kg of the extract for 15 consecutive days showed no alteration in the liver and kidney functions.",
keywords = "cytoprotective, H. pylori, liver and kidney functions, peptic ulcer, scopoletin, scopolin",
author = "Awaad, {Amani S.} and Al-Rifai, {Asmaa A.} and El-Meligy, {Reham M.} and Alafeefy, {Ahmed M.} and Zain, {Mohamed E.}",
year = "2015",
month = "9",
day = "1",
doi = "10.1002/ptr.5379",
language = "English",
volume = "29",
pages = "1311--1316",
journal = "Phytotherapy Research",
issn = "0951-418X",
publisher = "John Wiley and Sons Ltd",
number = "9",

}

New Activities for Isolated Compounds from Convolvulus austro-aegyptiacus as Anti-ulcerogenic, Anti-Helicobacter pylori and Their Mimic Synthesis Using Bio-guided Fractionation. / Awaad, Amani S.; Al-Rifai, Asmaa A.; El-Meligy, Reham M.; Alafeefy, Ahmed M.; Zain, Mohamed E.

In: Phytotherapy Research, Vol. 29, No. 9, 01.09.2015, p. 1311-1316.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - New Activities for Isolated Compounds from Convolvulus austro-aegyptiacus as Anti-ulcerogenic, Anti-Helicobacter pylori and Their Mimic Synthesis Using Bio-guided Fractionation

AU - Awaad, Amani S.

AU - Al-Rifai, Asmaa A.

AU - El-Meligy, Reham M.

AU - Alafeefy, Ahmed M.

AU - Zain, Mohamed E.

PY - 2015/9/1

Y1 - 2015/9/1

N2 - Bio-guided fractionation of the total alcoholic extract of Convolvulus austro-aegyptiacus was screened for its anti-ulcerogenic activity, using an absolute-ethanol-induced ulcer model at 500 and 1000mg/kg doses. Two compounds were isolated from the butanol extract of C. austro-aegyptiacus and identified by 1H and 13C nuclear magnetic resonance as scopoletin and scopolin. The isolated compounds (50mg/kg) showed a remarkable anti-ulcerogenic activity because they exhibited control-ulcer protection by 16.7% and 90.8%, respectively. The acute toxicity study showed that the extract is highly safe; the median lethal dose (LD50) was more than 4000mg/kg. Moreover, the obtained results were confirmed by the sub-chronic toxicity because the rats that have been administered 1000mg/kg of the extract for 15 consecutive days showed no alteration in the liver and kidney functions.

AB - Bio-guided fractionation of the total alcoholic extract of Convolvulus austro-aegyptiacus was screened for its anti-ulcerogenic activity, using an absolute-ethanol-induced ulcer model at 500 and 1000mg/kg doses. Two compounds were isolated from the butanol extract of C. austro-aegyptiacus and identified by 1H and 13C nuclear magnetic resonance as scopoletin and scopolin. The isolated compounds (50mg/kg) showed a remarkable anti-ulcerogenic activity because they exhibited control-ulcer protection by 16.7% and 90.8%, respectively. The acute toxicity study showed that the extract is highly safe; the median lethal dose (LD50) was more than 4000mg/kg. Moreover, the obtained results were confirmed by the sub-chronic toxicity because the rats that have been administered 1000mg/kg of the extract for 15 consecutive days showed no alteration in the liver and kidney functions.

KW - cytoprotective

KW - H. pylori

KW - liver and kidney functions

KW - peptic ulcer

KW - scopoletin

KW - scopolin

UR - http://www.scopus.com/inward/record.url?scp=84941175597&partnerID=8YFLogxK

U2 - 10.1002/ptr.5379

DO - 10.1002/ptr.5379

M3 - Article

VL - 29

SP - 1311

EP - 1316

JO - Phytotherapy Research

JF - Phytotherapy Research

SN - 0951-418X

IS - 9

ER -