Prophylactic role of a-lipoic acid and vitamin E against zinc oxide nanoparticles induced metabolic and immune disorders in rat's liver

N. M. Al-Rasheed, N. M. Al-Rasheed, N. A.Abdel Baky, L. M. Faddah, A. J. Fatani, I. H. Hasan, R. A. Mohamad

Research output: Contribution to journalReview articleResearchpeer-review

11 Citations (Scopus)

Abstract

OBJECTIVES: Potential health hazard is associated with the wide use of nanoparticles. The prophylactic role of either a-lipoic acid (a-lip) or vitamin E (vit E) against the toxic effect of zinc oxide nano-particles (ZnONPs) induced metabolic disorder, inflammation and DNA damage in rat livers was studied. MATERIALS AND METHODS: ZnO-NPs were administered orally using two doses (600 mg and 1 g/kg body weight/day for 5 conscutive days). Some biomarkers of tissue damage, metabolic disorder, and DNA damage were investigated to explore the protective mechanisms of a-lip or vit E against ZnO-NPs induced hepatotoxicity. RESULTS: Co-administration of either a-lip (200 mg/kg body weight) or vit E (100 mg/kg body weight) daily for three weeks to ZnO-NPs intoxicated rats, significantly down-modulated the marked increase in serum ALT (marker of liver damage) and also serum glucose level (marker of metabolic disorder) as well as the pro-inflammatory biomarkers including nitric oxide (NO), tumor necrosis factor-a (TNF-a), interleukin-6 (IL-6), C-reactive protein (CRP), and immunoglobin G (IGg). Reduced glutathione level was decreased while caspase3 level was elevated in liver tissues of ZnO-NPs treated group compared with intoxicated one. Moreover histopathological examination of liver tissue supported the previous biochemical markers. Furthermore, ZnO-NPs induced hepatic oxidative DNA damage. CONCLUSIONS: Either a-lip or vit E proved to be hepatoprotective agents against ZnONPs toxicity because they ameliorated metabolic and immune disorders related to liver damage and modulated the previous measured parameters.

Original languageEnglish
Pages (from-to)1813-1828
Number of pages16
JournalEuropean Review for Medical and Pharmacological Sciences
Volume18
Issue number12
Publication statusPublished - 1 Jan 2014

Fingerprint

Zinc Oxide
Thioctic Acid
Immune System Diseases
Vitamin E
Nanoparticles
Lip
Liver
Biomarkers
DNA Damage
Body Weight
Poisons
C-Reactive Protein
Glutathione
Interleukin-6
Nitric Oxide
Tumor Necrosis Factor-alpha
Inflammation
Glucose
Health
Serum

Keywords

  • A-lipoic acid
  • Deoxyribonucleic acid
  • Tumor necrosis factor-a
  • Vitamin E
  • Zinc oxide nano-particles

Cite this

Al-Rasheed, N. M. ; Al-Rasheed, N. M. ; Baky, N. A.Abdel ; Faddah, L. M. ; Fatani, A. J. ; Hasan, I. H. ; Mohamad, R. A. / Prophylactic role of a-lipoic acid and vitamin E against zinc oxide nanoparticles induced metabolic and immune disorders in rat's liver. In: European Review for Medical and Pharmacological Sciences. 2014 ; Vol. 18, No. 12. pp. 1813-1828.
@article{0f6de4d357724af994fd6086f3d248aa,
title = "Prophylactic role of a-lipoic acid and vitamin E against zinc oxide nanoparticles induced metabolic and immune disorders in rat's liver",
abstract = "OBJECTIVES: Potential health hazard is associated with the wide use of nanoparticles. The prophylactic role of either a-lipoic acid (a-lip) or vitamin E (vit E) against the toxic effect of zinc oxide nano-particles (ZnONPs) induced metabolic disorder, inflammation and DNA damage in rat livers was studied. MATERIALS AND METHODS: ZnO-NPs were administered orally using two doses (600 mg and 1 g/kg body weight/day for 5 conscutive days). Some biomarkers of tissue damage, metabolic disorder, and DNA damage were investigated to explore the protective mechanisms of a-lip or vit E against ZnO-NPs induced hepatotoxicity. RESULTS: Co-administration of either a-lip (200 mg/kg body weight) or vit E (100 mg/kg body weight) daily for three weeks to ZnO-NPs intoxicated rats, significantly down-modulated the marked increase in serum ALT (marker of liver damage) and also serum glucose level (marker of metabolic disorder) as well as the pro-inflammatory biomarkers including nitric oxide (NO), tumor necrosis factor-a (TNF-a), interleukin-6 (IL-6), C-reactive protein (CRP), and immunoglobin G (IGg). Reduced glutathione level was decreased while caspase3 level was elevated in liver tissues of ZnO-NPs treated group compared with intoxicated one. Moreover histopathological examination of liver tissue supported the previous biochemical markers. Furthermore, ZnO-NPs induced hepatic oxidative DNA damage. CONCLUSIONS: Either a-lip or vit E proved to be hepatoprotective agents against ZnONPs toxicity because they ameliorated metabolic and immune disorders related to liver damage and modulated the previous measured parameters.",
keywords = "A-lipoic acid, Deoxyribonucleic acid, Tumor necrosis factor-a, Vitamin E, Zinc oxide nano-particles",
author = "Al-Rasheed, {N. M.} and Al-Rasheed, {N. M.} and Baky, {N. A.Abdel} and Faddah, {L. M.} and Fatani, {A. J.} and Hasan, {I. H.} and Mohamad, {R. A.}",
year = "2014",
month = "1",
day = "1",
language = "English",
volume = "18",
pages = "1813--1828",
journal = "European Review for Medical and Pharmacological Sciences",
issn = "1128-3602",
publisher = "Verduci Editore",
number = "12",

}

Prophylactic role of a-lipoic acid and vitamin E against zinc oxide nanoparticles induced metabolic and immune disorders in rat's liver. / Al-Rasheed, N. M.; Al-Rasheed, N. M.; Baky, N. A.Abdel; Faddah, L. M.; Fatani, A. J.; Hasan, I. H.; Mohamad, R. A.

In: European Review for Medical and Pharmacological Sciences, Vol. 18, No. 12, 01.01.2014, p. 1813-1828.

Research output: Contribution to journalReview articleResearchpeer-review

TY - JOUR

T1 - Prophylactic role of a-lipoic acid and vitamin E against zinc oxide nanoparticles induced metabolic and immune disorders in rat's liver

AU - Al-Rasheed, N. M.

AU - Al-Rasheed, N. M.

AU - Baky, N. A.Abdel

AU - Faddah, L. M.

AU - Fatani, A. J.

AU - Hasan, I. H.

AU - Mohamad, R. A.

PY - 2014/1/1

Y1 - 2014/1/1

N2 - OBJECTIVES: Potential health hazard is associated with the wide use of nanoparticles. The prophylactic role of either a-lipoic acid (a-lip) or vitamin E (vit E) against the toxic effect of zinc oxide nano-particles (ZnONPs) induced metabolic disorder, inflammation and DNA damage in rat livers was studied. MATERIALS AND METHODS: ZnO-NPs were administered orally using two doses (600 mg and 1 g/kg body weight/day for 5 conscutive days). Some biomarkers of tissue damage, metabolic disorder, and DNA damage were investigated to explore the protective mechanisms of a-lip or vit E against ZnO-NPs induced hepatotoxicity. RESULTS: Co-administration of either a-lip (200 mg/kg body weight) or vit E (100 mg/kg body weight) daily for three weeks to ZnO-NPs intoxicated rats, significantly down-modulated the marked increase in serum ALT (marker of liver damage) and also serum glucose level (marker of metabolic disorder) as well as the pro-inflammatory biomarkers including nitric oxide (NO), tumor necrosis factor-a (TNF-a), interleukin-6 (IL-6), C-reactive protein (CRP), and immunoglobin G (IGg). Reduced glutathione level was decreased while caspase3 level was elevated in liver tissues of ZnO-NPs treated group compared with intoxicated one. Moreover histopathological examination of liver tissue supported the previous biochemical markers. Furthermore, ZnO-NPs induced hepatic oxidative DNA damage. CONCLUSIONS: Either a-lip or vit E proved to be hepatoprotective agents against ZnONPs toxicity because they ameliorated metabolic and immune disorders related to liver damage and modulated the previous measured parameters.

AB - OBJECTIVES: Potential health hazard is associated with the wide use of nanoparticles. The prophylactic role of either a-lipoic acid (a-lip) or vitamin E (vit E) against the toxic effect of zinc oxide nano-particles (ZnONPs) induced metabolic disorder, inflammation and DNA damage in rat livers was studied. MATERIALS AND METHODS: ZnO-NPs were administered orally using two doses (600 mg and 1 g/kg body weight/day for 5 conscutive days). Some biomarkers of tissue damage, metabolic disorder, and DNA damage were investigated to explore the protective mechanisms of a-lip or vit E against ZnO-NPs induced hepatotoxicity. RESULTS: Co-administration of either a-lip (200 mg/kg body weight) or vit E (100 mg/kg body weight) daily for three weeks to ZnO-NPs intoxicated rats, significantly down-modulated the marked increase in serum ALT (marker of liver damage) and also serum glucose level (marker of metabolic disorder) as well as the pro-inflammatory biomarkers including nitric oxide (NO), tumor necrosis factor-a (TNF-a), interleukin-6 (IL-6), C-reactive protein (CRP), and immunoglobin G (IGg). Reduced glutathione level was decreased while caspase3 level was elevated in liver tissues of ZnO-NPs treated group compared with intoxicated one. Moreover histopathological examination of liver tissue supported the previous biochemical markers. Furthermore, ZnO-NPs induced hepatic oxidative DNA damage. CONCLUSIONS: Either a-lip or vit E proved to be hepatoprotective agents against ZnONPs toxicity because they ameliorated metabolic and immune disorders related to liver damage and modulated the previous measured parameters.

KW - A-lipoic acid

KW - Deoxyribonucleic acid

KW - Tumor necrosis factor-a

KW - Vitamin E

KW - Zinc oxide nano-particles

UR - http://www.scopus.com/inward/record.url?scp=84922449490&partnerID=8YFLogxK

M3 - Review article

VL - 18

SP - 1813

EP - 1828

JO - European Review for Medical and Pharmacological Sciences

JF - European Review for Medical and Pharmacological Sciences

SN - 1128-3602

IS - 12

ER -