Risk factors and immune response to hepatitis E viral infection among acute hepatitis patients in Assiut, Egypt.

Salwa S Seif Eldin, Ismail Seddik, Enas A. Daef, M. T. Shata, Marwa Raafat, Laila Abdel Baky, M. A. Nafeh

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Abstract

Hepatitis E virus (HEV) infection is a common cause of acute viral hepatitis (AVH) in Egypt. We aimed to identify risk factors of HEV among acute hepatitis cases, measure HEV specific immune response to differentiate between symptomatic and asymptomatic infections. The study included symptomatic acute hepatitis (AH) patients (n = 235) and asymptomatic contacts (n = 200) to HEV cases. They completed a lifestyle questionnaire, screened for common hepatotropic viruses. Blood and serum samples were collected from patients and contacts after onset of disease and follow-up samples collected until convalescence. PBMC were separated and tested for specific HEV T-cell response by INF-gamma ELISPOT assay. Serum samples were tested for IgM and IgG anti-hepatitis E virus by ELISA. IgM antibodies to HAV were detected in 19 patients (8.1%), 37 (15.7%) with HBV, 10 (4.3%) with HCV. HEV infection was identified in 42 (16%) patients with AVH. Of the 200 contacts, 14 (7%) had serological evidence of recent HEV asymptomatic infection, showed stronger CMI responses than HEV infected subjects (2540 +/- 28 and 182 +/- 389 ISCs/106 cells, respectively; P < 0.05). In conclusion, HEV is a major cause of AVH in Egypt. Asymptomatic HEV patients are likely to have stronger immune responses including CMI responses, than symptomatic cases.

Original languageEnglish
Pages (from-to)73-86
Number of pages14
JournalThe Egyptian journal of immunology / Egyptian Association of Immunologists
Volume17
Issue number1
Publication statusPublished - 1 Dec 2010

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Hepatitis E
Hepatitis E virus
Egypt
Virus Diseases
Hepatitis
Asymptomatic Infections
Enzyme-Linked Immunospot Assay
Serum
Immunoglobulin M
Life Style

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Eldin, Salwa S Seif ; Seddik, Ismail ; Daef, Enas A. ; Shata, M. T. ; Raafat, Marwa ; Abdel Baky, Laila ; Nafeh, M. A. / Risk factors and immune response to hepatitis E viral infection among acute hepatitis patients in Assiut, Egypt. In: The Egyptian journal of immunology / Egyptian Association of Immunologists. 2010 ; Vol. 17, No. 1. pp. 73-86.
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abstract = "Hepatitis E virus (HEV) infection is a common cause of acute viral hepatitis (AVH) in Egypt. We aimed to identify risk factors of HEV among acute hepatitis cases, measure HEV specific immune response to differentiate between symptomatic and asymptomatic infections. The study included symptomatic acute hepatitis (AH) patients (n = 235) and asymptomatic contacts (n = 200) to HEV cases. They completed a lifestyle questionnaire, screened for common hepatotropic viruses. Blood and serum samples were collected from patients and contacts after onset of disease and follow-up samples collected until convalescence. PBMC were separated and tested for specific HEV T-cell response by INF-gamma ELISPOT assay. Serum samples were tested for IgM and IgG anti-hepatitis E virus by ELISA. IgM antibodies to HAV were detected in 19 patients (8.1{\%}), 37 (15.7{\%}) with HBV, 10 (4.3{\%}) with HCV. HEV infection was identified in 42 (16{\%}) patients with AVH. Of the 200 contacts, 14 (7{\%}) had serological evidence of recent HEV asymptomatic infection, showed stronger CMI responses than HEV infected subjects (2540 +/- 28 and 182 +/- 389 ISCs/106 cells, respectively; P < 0.05). In conclusion, HEV is a major cause of AVH in Egypt. Asymptomatic HEV patients are likely to have stronger immune responses including CMI responses, than symptomatic cases.",
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Risk factors and immune response to hepatitis E viral infection among acute hepatitis patients in Assiut, Egypt. / Eldin, Salwa S Seif; Seddik, Ismail; Daef, Enas A.; Shata, M. T.; Raafat, Marwa; Abdel Baky, Laila; Nafeh, M. A.

In: The Egyptian journal of immunology / Egyptian Association of Immunologists, Vol. 17, No. 1, 01.12.2010, p. 73-86.

Research output: Contribution to journalArticleResearchpeer-review

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