Role of intestinal microflora (Lactobacillus Acidophilus) in phagocytic function of leukocytes in type 2 diabetic patients

Fawzia H. Abo Ali, Zeinab A. Ashour, Rasha Y. Shahin, Wafaa K. Zaki, Salwa B. Ragab, Mohamed Y. Attia

Research output: Contribution to journalArticleResearchpeer-review

4 Citations (Scopus)

Abstract

The prevalence of obesity, insulin resistance and type 2 diabetes has steadily increased in the last decades. In addition to the genetic and environmental factors, gut microbiota may play an important role in the modulation of intermediary phenotypes leading to metabolic disease. Infection is an important cause of morbidity and mortality in diabetic patients. Chronic hyperglycemia impairs host defense mechanism such as cell mediated immunity, polymorphonuclear leukocyte function, and antibody formation. So we aimed to study the association between intestinal microflora (Lactobacillus acidophilus) count and phagocytic activity of polymorphonuclear leukocytes in humans with type 2 diabetes.The study included 20 type 2 diabetic patients with good glycemic control and 20 type 2 diabetic patients with poor glycemic control. In addition, 20 normal healthy subjects were included as normal controls. The fecal composition of L. acidophilus was detected using de Man Rogosa Sharp agar followed by further confirmation using the polymerase chain reaction technique. Phagocytic function of polymorphonuclear leukocytes was assessed using the phagocytosis index %. Fecal L. acidophilus count was significantly increased among uncontrolled diabetic patients, while the phagocytosis index % was significantly reduced among the same patients. In uncontrolled diabetics, a significant positive correlation was observed between fecal L. acidophilus count and HbA1c and a significant negative correlation between phagocytic activity and L. acidophilus count. In conclusion, type 2 diabetes is associated with compositional changes in fecal L. acidophilus especially in the uncontrolled diabetes. The levels of glucose tolerance or severity of diabetes should be considered while linking the level of intestinal microbiota with a phagocytosis index of leukocytes.

Original languageEnglish
Pages (from-to)95-101
Number of pages7
JournalEgyptian Journal of Medical Human Genetics
Volume14
Issue number1
DOIs
Publication statusPublished - 1 Jan 2013

Fingerprint

Lactobacillus acidophilus
Leukocytes
Phagocytosis
Type 2 Diabetes Mellitus
Neutrophils
Metabolic Diseases
Cellular Immunity
Hyperglycemia
Agar
Antibody Formation
Insulin Resistance
Gastrointestinal Microbiome
Healthy Volunteers
Obesity
Morbidity
Phenotype
Glucose
Polymerase Chain Reaction
Mortality
Infection

Keywords

  • HbA1c
  • Lactobacillus acidophilus
  • Phagocytosis index %
  • Type 2 diabetes

Cite this

Abo Ali, Fawzia H. ; Ashour, Zeinab A. ; Shahin, Rasha Y. ; Zaki, Wafaa K. ; Ragab, Salwa B. ; Attia, Mohamed Y. / Role of intestinal microflora (Lactobacillus Acidophilus) in phagocytic function of leukocytes in type 2 diabetic patients. In: Egyptian Journal of Medical Human Genetics. 2013 ; Vol. 14, No. 1. pp. 95-101.
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Role of intestinal microflora (Lactobacillus Acidophilus) in phagocytic function of leukocytes in type 2 diabetic patients. / Abo Ali, Fawzia H.; Ashour, Zeinab A.; Shahin, Rasha Y.; Zaki, Wafaa K.; Ragab, Salwa B.; Attia, Mohamed Y.

In: Egyptian Journal of Medical Human Genetics, Vol. 14, No. 1, 01.01.2013, p. 95-101.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Abo Ali, Fawzia H.

AU - Ashour, Zeinab A.

AU - Shahin, Rasha Y.

AU - Zaki, Wafaa K.

AU - Ragab, Salwa B.

AU - Attia, Mohamed Y.

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AB - The prevalence of obesity, insulin resistance and type 2 diabetes has steadily increased in the last decades. In addition to the genetic and environmental factors, gut microbiota may play an important role in the modulation of intermediary phenotypes leading to metabolic disease. Infection is an important cause of morbidity and mortality in diabetic patients. Chronic hyperglycemia impairs host defense mechanism such as cell mediated immunity, polymorphonuclear leukocyte function, and antibody formation. So we aimed to study the association between intestinal microflora (Lactobacillus acidophilus) count and phagocytic activity of polymorphonuclear leukocytes in humans with type 2 diabetes.The study included 20 type 2 diabetic patients with good glycemic control and 20 type 2 diabetic patients with poor glycemic control. In addition, 20 normal healthy subjects were included as normal controls. The fecal composition of L. acidophilus was detected using de Man Rogosa Sharp agar followed by further confirmation using the polymerase chain reaction technique. Phagocytic function of polymorphonuclear leukocytes was assessed using the phagocytosis index %. Fecal L. acidophilus count was significantly increased among uncontrolled diabetic patients, while the phagocytosis index % was significantly reduced among the same patients. In uncontrolled diabetics, a significant positive correlation was observed between fecal L. acidophilus count and HbA1c and a significant negative correlation between phagocytic activity and L. acidophilus count. In conclusion, type 2 diabetes is associated with compositional changes in fecal L. acidophilus especially in the uncontrolled diabetes. The levels of glucose tolerance or severity of diabetes should be considered while linking the level of intestinal microbiota with a phagocytosis index of leukocytes.

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