Role of natural antioxidants in the modulation of plasma amino acid pattern in rats exposed to hemic hypoxia

Nouf Mohamed Al-Rasheed, Laila Mohamed Fadda, Nawal Mohamed Al-Rasheed, Hala Attia, Hanaa Mahmoud Ali, Hanaa El-Agami

Research output: Contribution to journalArticleResearchpeer-review

Abstract

The aim of this work was to investigate whether the free radical scavengers, L-arginine (L-arg) and/or carnosine, either alone, or in combination would modulate tissue injury induced by hypoxia by measuring Fischer's ratio [concentrations of branched chain amino acids (BCAAs)/aromatic amino acids]. Decreased Fischer's ratios and increased malondialdehyde (MDA) led to pathogeneses of many diseases. Rats were injected with sodium nitrite (60 mg/kg) to establish hypoxia. They were treated with L-arg, (200 mg/ kg) and/or carnosine (200 mg/ kg) and their combination 24 and 1 h prior to sodium nitrite intoxication. The results revealed that hypoxia significantly decreased hemoglobin, arginine, citrulline and proline and increased sLDH, MDA , ammonia , urea, BCAAs (valine, leucine and isoleucine) and aromatic amino acids (phenylalanine and tyrosine ). The Fischer's ratio was decreased compared with the control; the administration of the aforementioned antioxidants ameliorated most of the previously altered parameters. It was concluded that Fischer's ratio was a valuable tool for understanding the pathology of hemic hypoxia, evaluating the degree of the modulatory effect of various natural antioxidants and the synergy between L-arg and carnosine in ameliorating the effect of sodium nitrite on amino acids pattern. Thus, it could be recommended to administer the combination of L-arg and carnosine in the areas of high altitudes to combat the hazard effect of hypoxia on hemoglobin concentration and MDA level.

Original languageEnglish
Pages (from-to)741-749
Number of pages9
JournalBrazilian Archives of Biology and Technology
Volume58
Issue number5
DOIs
Publication statusPublished - 1 Jan 2015

Fingerprint

carnosine
hypoxia
sodium nitrite
antioxidants
amino acids
malondialdehyde
rats
blood
branched chain amino acids
arginine
aromatic compounds
hemoglobin
citrulline
free radical scavengers
isoleucine
valine
phenylalanine
leucine
tyrosine
proline

Keywords

  • Amino acids
  • Carnosine
  • Fisher's ratio
  • L-arginine
  • Natural antioxidants
  • Urea

Cite this

Al-Rasheed, Nouf Mohamed ; Fadda, Laila Mohamed ; Al-Rasheed, Nawal Mohamed ; Attia, Hala ; Ali, Hanaa Mahmoud ; El-Agami, Hanaa. / Role of natural antioxidants in the modulation of plasma amino acid pattern in rats exposed to hemic hypoxia. In: Brazilian Archives of Biology and Technology. 2015 ; Vol. 58, No. 5. pp. 741-749.
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Role of natural antioxidants in the modulation of plasma amino acid pattern in rats exposed to hemic hypoxia. / Al-Rasheed, Nouf Mohamed; Fadda, Laila Mohamed; Al-Rasheed, Nawal Mohamed; Attia, Hala; Ali, Hanaa Mahmoud; El-Agami, Hanaa.

In: Brazilian Archives of Biology and Technology, Vol. 58, No. 5, 01.01.2015, p. 741-749.

Research output: Contribution to journalArticleResearchpeer-review

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