Stability indicating methods for the determination of some anti-fungal agents using densitometric and RP-HPLC methods

Bahia Abbas Mousa, Naglaa Mahmoud El-Kousy, Ramzia Ismail El-Bagary, Nashwah Gadalla Mohamed

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Two chromatographic methods were developed for the determination of some anti-fungal drugs in the presence of either their degradation products or cortisone derivatives. The densitometric method determined mixtures of each of ketoconazole (KT), clotrimazole (CL), miconazole nitrate (MN) and econazole nitrate (EN) with the degradation products of each one. Mixtures of MN with hydrocortisone (HC) and of EN with triamcinolone acetonide (TA) were also successfully separated and determined by this technique. For KT and CL, a mixture of methanol:water:triethylamine (70:28:2 v/v) was used as a developing system and the spots were scanned at 243 nm and 220 nm for KT and CL, respectively. For MN and EN, a mixture of hexane: isopropyl alcohol: triethylamine (80:17:3 v/v) was used as a developing system and the spots were scanned at 225 nm for both drugs. The HPLC method determined mixtures of CL or EN with their degradation products which were separated and quantified on a Zorbax C8 column. Elution was carried out using methanol:phosphate buffer pH 2.5 (65:35 v/v) as a mobile phase at a flow rate of 1.5 ml/min and UV detection at 220 nm for CL. For EN, a mixture of methanol:water containing 0.06 ml triethylamine pH 10 (75:25 v/v) was used as a mobile phase at a flow rate of 1.5 ml/min and UV detection at 225 nm. The methods were also used to separate mixtures of CL with betamethasone dipropionate (BD) and EN with TA in a laboratory prepared mixture and in pharmaceutical preparations. The methods were sensitive, precise and applicable for determination of the drugs in pharmaceutical dosage forms.

Original languageEnglish
Pages (from-to)143-149
Number of pages7
JournalChemical and Pharmaceutical Bulletin
Volume56
Issue number2
DOIs
Publication statusPublished - 1 Feb 2008

Fingerprint

Antifungal agents
Econazole
Clotrimazole
High Pressure Liquid Chromatography
Miconazole
Ketoconazole
Methanol
Triamcinolone Acetonide
Pharmaceutical Preparations
Degradation
2-Propanol
Water
Cortisone
Flow rate
Dosage Forms
Hexanes
Hydrocortisone
Buffers
Phosphates
Derivatives

Keywords

  • Clotrimazole
  • Densitometric
  • Econazole nitrate
  • HPLC
  • Ketoconazole
  • Miconazole nitrate

Cite this

@article{e574f688ecdc4958a4fb9163536d46c4,
title = "Stability indicating methods for the determination of some anti-fungal agents using densitometric and RP-HPLC methods",
abstract = "Two chromatographic methods were developed for the determination of some anti-fungal drugs in the presence of either their degradation products or cortisone derivatives. The densitometric method determined mixtures of each of ketoconazole (KT), clotrimazole (CL), miconazole nitrate (MN) and econazole nitrate (EN) with the degradation products of each one. Mixtures of MN with hydrocortisone (HC) and of EN with triamcinolone acetonide (TA) were also successfully separated and determined by this technique. For KT and CL, a mixture of methanol:water:triethylamine (70:28:2 v/v) was used as a developing system and the spots were scanned at 243 nm and 220 nm for KT and CL, respectively. For MN and EN, a mixture of hexane: isopropyl alcohol: triethylamine (80:17:3 v/v) was used as a developing system and the spots were scanned at 225 nm for both drugs. The HPLC method determined mixtures of CL or EN with their degradation products which were separated and quantified on a Zorbax C8 column. Elution was carried out using methanol:phosphate buffer pH 2.5 (65:35 v/v) as a mobile phase at a flow rate of 1.5 ml/min and UV detection at 220 nm for CL. For EN, a mixture of methanol:water containing 0.06 ml triethylamine pH 10 (75:25 v/v) was used as a mobile phase at a flow rate of 1.5 ml/min and UV detection at 225 nm. The methods were also used to separate mixtures of CL with betamethasone dipropionate (BD) and EN with TA in a laboratory prepared mixture and in pharmaceutical preparations. The methods were sensitive, precise and applicable for determination of the drugs in pharmaceutical dosage forms.",
keywords = "Clotrimazole, Densitometric, Econazole nitrate, HPLC, Ketoconazole, Miconazole nitrate",
author = "Mousa, {Bahia Abbas} and El-Kousy, {Naglaa Mahmoud} and El-Bagary, {Ramzia Ismail} and Mohamed, {Nashwah Gadalla}",
year = "2008",
month = "2",
day = "1",
doi = "10.1248/cpb.56.143",
language = "English",
volume = "56",
pages = "143--149",
journal = "Chemical and Pharmaceutical Bulletin",
issn = "0009-2363",
publisher = "Pharmaceutical Society of Japan",
number = "2",

}

Stability indicating methods for the determination of some anti-fungal agents using densitometric and RP-HPLC methods. / Mousa, Bahia Abbas; El-Kousy, Naglaa Mahmoud; El-Bagary, Ramzia Ismail; Mohamed, Nashwah Gadalla.

In: Chemical and Pharmaceutical Bulletin, Vol. 56, No. 2, 01.02.2008, p. 143-149.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Stability indicating methods for the determination of some anti-fungal agents using densitometric and RP-HPLC methods

AU - Mousa, Bahia Abbas

AU - El-Kousy, Naglaa Mahmoud

AU - El-Bagary, Ramzia Ismail

AU - Mohamed, Nashwah Gadalla

PY - 2008/2/1

Y1 - 2008/2/1

N2 - Two chromatographic methods were developed for the determination of some anti-fungal drugs in the presence of either their degradation products or cortisone derivatives. The densitometric method determined mixtures of each of ketoconazole (KT), clotrimazole (CL), miconazole nitrate (MN) and econazole nitrate (EN) with the degradation products of each one. Mixtures of MN with hydrocortisone (HC) and of EN with triamcinolone acetonide (TA) were also successfully separated and determined by this technique. For KT and CL, a mixture of methanol:water:triethylamine (70:28:2 v/v) was used as a developing system and the spots were scanned at 243 nm and 220 nm for KT and CL, respectively. For MN and EN, a mixture of hexane: isopropyl alcohol: triethylamine (80:17:3 v/v) was used as a developing system and the spots were scanned at 225 nm for both drugs. The HPLC method determined mixtures of CL or EN with their degradation products which were separated and quantified on a Zorbax C8 column. Elution was carried out using methanol:phosphate buffer pH 2.5 (65:35 v/v) as a mobile phase at a flow rate of 1.5 ml/min and UV detection at 220 nm for CL. For EN, a mixture of methanol:water containing 0.06 ml triethylamine pH 10 (75:25 v/v) was used as a mobile phase at a flow rate of 1.5 ml/min and UV detection at 225 nm. The methods were also used to separate mixtures of CL with betamethasone dipropionate (BD) and EN with TA in a laboratory prepared mixture and in pharmaceutical preparations. The methods were sensitive, precise and applicable for determination of the drugs in pharmaceutical dosage forms.

AB - Two chromatographic methods were developed for the determination of some anti-fungal drugs in the presence of either their degradation products or cortisone derivatives. The densitometric method determined mixtures of each of ketoconazole (KT), clotrimazole (CL), miconazole nitrate (MN) and econazole nitrate (EN) with the degradation products of each one. Mixtures of MN with hydrocortisone (HC) and of EN with triamcinolone acetonide (TA) were also successfully separated and determined by this technique. For KT and CL, a mixture of methanol:water:triethylamine (70:28:2 v/v) was used as a developing system and the spots were scanned at 243 nm and 220 nm for KT and CL, respectively. For MN and EN, a mixture of hexane: isopropyl alcohol: triethylamine (80:17:3 v/v) was used as a developing system and the spots were scanned at 225 nm for both drugs. The HPLC method determined mixtures of CL or EN with their degradation products which were separated and quantified on a Zorbax C8 column. Elution was carried out using methanol:phosphate buffer pH 2.5 (65:35 v/v) as a mobile phase at a flow rate of 1.5 ml/min and UV detection at 220 nm for CL. For EN, a mixture of methanol:water containing 0.06 ml triethylamine pH 10 (75:25 v/v) was used as a mobile phase at a flow rate of 1.5 ml/min and UV detection at 225 nm. The methods were also used to separate mixtures of CL with betamethasone dipropionate (BD) and EN with TA in a laboratory prepared mixture and in pharmaceutical preparations. The methods were sensitive, precise and applicable for determination of the drugs in pharmaceutical dosage forms.

KW - Clotrimazole

KW - Densitometric

KW - Econazole nitrate

KW - HPLC

KW - Ketoconazole

KW - Miconazole nitrate

UR - http://www.scopus.com/inward/record.url?scp=38849098529&partnerID=8YFLogxK

U2 - 10.1248/cpb.56.143

DO - 10.1248/cpb.56.143

M3 - Article

C2 - 18239297

AN - SCOPUS:38849098529

VL - 56

SP - 143

EP - 149

JO - Chemical and Pharmaceutical Bulletin

JF - Chemical and Pharmaceutical Bulletin

SN - 0009-2363

IS - 2

ER -