Study of some proinflammatory and antiinflammatory cytokines in atopic and nonatopic asthmatics

Aly A. Haggag, Nahla E. El-Ashmawy, Fawzy El-Emery, Amal Abou Kamar, Gamal A. El-Azab

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Asthma is a disease of the airways characterized by chronic inflammation with infiltration of lymphocytes, eosinophils, and mast cells together with epithelial desquamation, thickening and disorganization of tissues of the airway wall. Cytokines produced by T-lymphocytes are important immuno-modulatory agents that regulate the inflammation of the airways of asthmatic patients. The aim of the present study was to estimate the level of the pro-inflammatory cytokine interleukin-2 (IL-2), and the anti-inflammatory cytokine IL-10 and their messenger RNA (mRNA) expression in the bronchoalveolar lavage (BAL) and peripheral blood of atopic and non-atopic asthmatics in order to elucidate their role in the pathogenesis of asthma. The study was conducted on 30 subjects divided into 3 groups of 10-persons each; group I (control group), group II (nonatopic asthmatics) and group III (atopic asthmatics). All asthmatics met the criteria of the American Thoracic Society of Asthma. IL-2 and IL-10 were measured by ELISA and the mRNA encoding each cytokine was detected using reverse-transcriptase polymerase chain reaction (RT-PCR). BAL and peripheral blood monocytes obtained from both atopic and non-atopic asthmatics showed an elevated level of IL-2 and a decreased level of IL-10 compared to the controls. RT-PCR detected IL-2 mRNA in BAL T-cells of both asthmatic groups and in peripheral blood monocytes of atopic asthmatics, but not in the non-asthmatic controls. On the other hand, IL-10 mRNA was expressed in BAL T-cells of atopic asthmatics and non-atopic asthmatics but in lower amount than it was in the non-asthmatic controls. Based on the findings of the present study, it could be speculated that diminished IL-10 production in asthma patients would lead to the unopposed synthesis of the pro-inflammatory cytokine IL-2 which contributes to the development and severity of asthma. Agents that modulate cytokine activity may play a role in controlling asthma.

Original languageEnglish
Pages (from-to)98-105
Number of pages8
JournalSaudi Pharmaceutical Journal
Volume10
Issue number3
Publication statusPublished - 1 Jan 2002

Fingerprint

Anti-Inflammatory Agents
Interleukin-10
Asthma
Interleukin-2
Bronchoalveolar Lavage
Cytokines
Messenger RNA
Reverse Transcriptase Polymerase Chain Reaction
T-Lymphocytes
Monocytes
Inflammation
Eosinophils
Mast Cells
Enzyme-Linked Immunosorbent Assay
Lymphocytes
Control Groups

Keywords

  • Bronchial asthma
  • Bronchoalveolar lavage
  • Interleukin-10
  • Interleukin-2
  • Peripheral blood monocytes

Cite this

Haggag, A. A., El-Ashmawy, N. E., El-Emery, F., Kamar, A. A., & El-Azab, G. A. (2002). Study of some proinflammatory and antiinflammatory cytokines in atopic and nonatopic asthmatics. Saudi Pharmaceutical Journal, 10(3), 98-105.
Haggag, Aly A. ; El-Ashmawy, Nahla E. ; El-Emery, Fawzy ; Kamar, Amal Abou ; El-Azab, Gamal A. / Study of some proinflammatory and antiinflammatory cytokines in atopic and nonatopic asthmatics. In: Saudi Pharmaceutical Journal. 2002 ; Vol. 10, No. 3. pp. 98-105.
@article{df712f42f4264d769f9861b4e8eac7ea,
title = "Study of some proinflammatory and antiinflammatory cytokines in atopic and nonatopic asthmatics",
abstract = "Asthma is a disease of the airways characterized by chronic inflammation with infiltration of lymphocytes, eosinophils, and mast cells together with epithelial desquamation, thickening and disorganization of tissues of the airway wall. Cytokines produced by T-lymphocytes are important immuno-modulatory agents that regulate the inflammation of the airways of asthmatic patients. The aim of the present study was to estimate the level of the pro-inflammatory cytokine interleukin-2 (IL-2), and the anti-inflammatory cytokine IL-10 and their messenger RNA (mRNA) expression in the bronchoalveolar lavage (BAL) and peripheral blood of atopic and non-atopic asthmatics in order to elucidate their role in the pathogenesis of asthma. The study was conducted on 30 subjects divided into 3 groups of 10-persons each; group I (control group), group II (nonatopic asthmatics) and group III (atopic asthmatics). All asthmatics met the criteria of the American Thoracic Society of Asthma. IL-2 and IL-10 were measured by ELISA and the mRNA encoding each cytokine was detected using reverse-transcriptase polymerase chain reaction (RT-PCR). BAL and peripheral blood monocytes obtained from both atopic and non-atopic asthmatics showed an elevated level of IL-2 and a decreased level of IL-10 compared to the controls. RT-PCR detected IL-2 mRNA in BAL T-cells of both asthmatic groups and in peripheral blood monocytes of atopic asthmatics, but not in the non-asthmatic controls. On the other hand, IL-10 mRNA was expressed in BAL T-cells of atopic asthmatics and non-atopic asthmatics but in lower amount than it was in the non-asthmatic controls. Based on the findings of the present study, it could be speculated that diminished IL-10 production in asthma patients would lead to the unopposed synthesis of the pro-inflammatory cytokine IL-2 which contributes to the development and severity of asthma. Agents that modulate cytokine activity may play a role in controlling asthma.",
keywords = "Bronchial asthma, Bronchoalveolar lavage, Interleukin-10, Interleukin-2, Peripheral blood monocytes",
author = "Haggag, {Aly A.} and El-Ashmawy, {Nahla E.} and Fawzy El-Emery and Kamar, {Amal Abou} and El-Azab, {Gamal A.}",
year = "2002",
month = "1",
day = "1",
language = "English",
volume = "10",
pages = "98--105",
journal = "Saudi Pharmaceutical Journal",
issn = "1319-0164",
publisher = "King Saud University",
number = "3",

}

Haggag, AA, El-Ashmawy, NE, El-Emery, F, Kamar, AA & El-Azab, GA 2002, 'Study of some proinflammatory and antiinflammatory cytokines in atopic and nonatopic asthmatics', Saudi Pharmaceutical Journal, vol. 10, no. 3, pp. 98-105.

Study of some proinflammatory and antiinflammatory cytokines in atopic and nonatopic asthmatics. / Haggag, Aly A.; El-Ashmawy, Nahla E.; El-Emery, Fawzy; Kamar, Amal Abou; El-Azab, Gamal A.

In: Saudi Pharmaceutical Journal, Vol. 10, No. 3, 01.01.2002, p. 98-105.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Study of some proinflammatory and antiinflammatory cytokines in atopic and nonatopic asthmatics

AU - Haggag, Aly A.

AU - El-Ashmawy, Nahla E.

AU - El-Emery, Fawzy

AU - Kamar, Amal Abou

AU - El-Azab, Gamal A.

PY - 2002/1/1

Y1 - 2002/1/1

N2 - Asthma is a disease of the airways characterized by chronic inflammation with infiltration of lymphocytes, eosinophils, and mast cells together with epithelial desquamation, thickening and disorganization of tissues of the airway wall. Cytokines produced by T-lymphocytes are important immuno-modulatory agents that regulate the inflammation of the airways of asthmatic patients. The aim of the present study was to estimate the level of the pro-inflammatory cytokine interleukin-2 (IL-2), and the anti-inflammatory cytokine IL-10 and their messenger RNA (mRNA) expression in the bronchoalveolar lavage (BAL) and peripheral blood of atopic and non-atopic asthmatics in order to elucidate their role in the pathogenesis of asthma. The study was conducted on 30 subjects divided into 3 groups of 10-persons each; group I (control group), group II (nonatopic asthmatics) and group III (atopic asthmatics). All asthmatics met the criteria of the American Thoracic Society of Asthma. IL-2 and IL-10 were measured by ELISA and the mRNA encoding each cytokine was detected using reverse-transcriptase polymerase chain reaction (RT-PCR). BAL and peripheral blood monocytes obtained from both atopic and non-atopic asthmatics showed an elevated level of IL-2 and a decreased level of IL-10 compared to the controls. RT-PCR detected IL-2 mRNA in BAL T-cells of both asthmatic groups and in peripheral blood monocytes of atopic asthmatics, but not in the non-asthmatic controls. On the other hand, IL-10 mRNA was expressed in BAL T-cells of atopic asthmatics and non-atopic asthmatics but in lower amount than it was in the non-asthmatic controls. Based on the findings of the present study, it could be speculated that diminished IL-10 production in asthma patients would lead to the unopposed synthesis of the pro-inflammatory cytokine IL-2 which contributes to the development and severity of asthma. Agents that modulate cytokine activity may play a role in controlling asthma.

AB - Asthma is a disease of the airways characterized by chronic inflammation with infiltration of lymphocytes, eosinophils, and mast cells together with epithelial desquamation, thickening and disorganization of tissues of the airway wall. Cytokines produced by T-lymphocytes are important immuno-modulatory agents that regulate the inflammation of the airways of asthmatic patients. The aim of the present study was to estimate the level of the pro-inflammatory cytokine interleukin-2 (IL-2), and the anti-inflammatory cytokine IL-10 and their messenger RNA (mRNA) expression in the bronchoalveolar lavage (BAL) and peripheral blood of atopic and non-atopic asthmatics in order to elucidate their role in the pathogenesis of asthma. The study was conducted on 30 subjects divided into 3 groups of 10-persons each; group I (control group), group II (nonatopic asthmatics) and group III (atopic asthmatics). All asthmatics met the criteria of the American Thoracic Society of Asthma. IL-2 and IL-10 were measured by ELISA and the mRNA encoding each cytokine was detected using reverse-transcriptase polymerase chain reaction (RT-PCR). BAL and peripheral blood monocytes obtained from both atopic and non-atopic asthmatics showed an elevated level of IL-2 and a decreased level of IL-10 compared to the controls. RT-PCR detected IL-2 mRNA in BAL T-cells of both asthmatic groups and in peripheral blood monocytes of atopic asthmatics, but not in the non-asthmatic controls. On the other hand, IL-10 mRNA was expressed in BAL T-cells of atopic asthmatics and non-atopic asthmatics but in lower amount than it was in the non-asthmatic controls. Based on the findings of the present study, it could be speculated that diminished IL-10 production in asthma patients would lead to the unopposed synthesis of the pro-inflammatory cytokine IL-2 which contributes to the development and severity of asthma. Agents that modulate cytokine activity may play a role in controlling asthma.

KW - Bronchial asthma

KW - Bronchoalveolar lavage

KW - Interleukin-10

KW - Interleukin-2

KW - Peripheral blood monocytes

UR - http://www.scopus.com/inward/record.url?scp=0036391059&partnerID=8YFLogxK

M3 - Article

VL - 10

SP - 98

EP - 105

JO - Saudi Pharmaceutical Journal

JF - Saudi Pharmaceutical Journal

SN - 1319-0164

IS - 3

ER -