The diagnostic impact of C4d, CD68, and NF-κB expression in the differentiation between recurrent Hepatitis C and acute cellular rejection after liver transplantation

Asmaa G. Abdou, Nancy Y. Asaad, Nermin Ehsan, Sheren Younes, Asmaa I. Gomaa, Walaa Elgendy

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6 Citations (Scopus)

Abstract

Liver transplantation is the selected treatment for patients with advanced liver disease and cirrhosis, mostly as a complication of hepatitis C virus (HCV). Recurrent HCV and acute cellular rejection (ACR) of the graft are the most common causes of graft failure. The distinction between the 2 conditions is essential because they are managed differently. In some cases, the clinical and histopathologic features may overlap between recurrent hepatitis C and ACR, making differentiation difficult. The aim of this study was to investigate the role of C4d, CD68, and nuclear factor kappa-B (NF-κB) in the differentiation between ACR and recurrent HCV in the post-liver-transplant biopsy using immunohistochemistry. C4d expression in endothelial cells of portal or central veins (P=0.001) and the number of macrophages highlighted by CD68 (P=0.02) were in favor of ACR, whereas NF-κB expression by hepatocytes was in favor of recurrent hepatitis C. Vascular injury demonstrated by endothelial expression of C4d and prominent macrophage infiltration identified by CD68 expression were the distinguishing criteria for ACR and representing humoral and cellular-mediated immunity as evoking factors for graft injury. The upregulation of NF-κB in the hepatocytes of recurrent hepatitis C could be an immune response to infection or it may be induced by HCV itself.

Original languageEnglish
Pages (from-to)639-647
Number of pages9
JournalApplied Immunohistochemistry and Molecular Morphology
Volume24
Issue number9
DOIs
Publication statusPublished - 1 Oct 2016

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NF-kappa B
Hepatitis C
Hepacivirus
Liver Transplantation
Transplants
Hepatocytes
Macrophages
Vascular System Injuries
Graft Rejection
Cellular Immunity
Liver Cirrhosis
Liver Diseases
Veins
Up-Regulation
Endothelial Cells
Immunohistochemistry
Biopsy
Liver
Wounds and Injuries
Infection

Keywords

  • ACR
  • C4d
  • CD68
  • liver graft failure
  • NF-κB
  • recurrent HCV

Cite this

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title = "The diagnostic impact of C4d, CD68, and NF-κB expression in the differentiation between recurrent Hepatitis C and acute cellular rejection after liver transplantation",
abstract = "Liver transplantation is the selected treatment for patients with advanced liver disease and cirrhosis, mostly as a complication of hepatitis C virus (HCV). Recurrent HCV and acute cellular rejection (ACR) of the graft are the most common causes of graft failure. The distinction between the 2 conditions is essential because they are managed differently. In some cases, the clinical and histopathologic features may overlap between recurrent hepatitis C and ACR, making differentiation difficult. The aim of this study was to investigate the role of C4d, CD68, and nuclear factor kappa-B (NF-κB) in the differentiation between ACR and recurrent HCV in the post-liver-transplant biopsy using immunohistochemistry. C4d expression in endothelial cells of portal or central veins (P=0.001) and the number of macrophages highlighted by CD68 (P=0.02) were in favor of ACR, whereas NF-κB expression by hepatocytes was in favor of recurrent hepatitis C. Vascular injury demonstrated by endothelial expression of C4d and prominent macrophage infiltration identified by CD68 expression were the distinguishing criteria for ACR and representing humoral and cellular-mediated immunity as evoking factors for graft injury. The upregulation of NF-κB in the hepatocytes of recurrent hepatitis C could be an immune response to infection or it may be induced by HCV itself.",
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The diagnostic impact of C4d, CD68, and NF-κB expression in the differentiation between recurrent Hepatitis C and acute cellular rejection after liver transplantation. / Abdou, Asmaa G.; Asaad, Nancy Y.; Ehsan, Nermin; Younes, Sheren; Gomaa, Asmaa I.; Elgendy, Walaa.

In: Applied Immunohistochemistry and Molecular Morphology, Vol. 24, No. 9, 01.10.2016, p. 639-647.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - The diagnostic impact of C4d, CD68, and NF-κB expression in the differentiation between recurrent Hepatitis C and acute cellular rejection after liver transplantation

AU - Abdou, Asmaa G.

AU - Asaad, Nancy Y.

AU - Ehsan, Nermin

AU - Younes, Sheren

AU - Gomaa, Asmaa I.

AU - Elgendy, Walaa

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