The efficacy of HGAL and LMO2 in the separation of lymphomas derived from small B cells in nodal and extranodal sites, including the bone marrow

Sheren F. Younes, Andrew H. Beck, Robert S. Ohgami, Izidore S. Lossos, Ronald Levy, Roger A. Warnke, Yasodha Natkunam

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Abstract

We studied the efficacy of 2 germinal center B-cell markers, HGAL and LMO2, in the separation of lymphomas derived from small B cells, particularly follicular lymphoma (FL) and marginal zone lymphoma occurring in nodal, extranodal, splenic, and bone marrow sites using immunohistochemical analysis for CD10, BCL6, BCL2, HGAL, and LMO2. Our results showed that HGAL and LMO2 are sensitive and specific markers for detecting FL in nodal and extranodal sites. In contrast, all markers were downregulated in FL infiltrates in the bone marrow. CD10 and HGAL were expressed in a subset of FLs in the bone marrow and were highly correlated with each other and with CD21, a marker of follicular dendritic cells. We conclude that HGAL and LMO2 should be considered in immunohistochemical panels used for the routine workup of lymphomas derived from small B cells. In the bone marrow, staining for HGAL or CD10 can be helpful in making a diagnosis of FL, although they are absent in a subset of cases.

Original languageEnglish
Pages (from-to)697-708
Number of pages12
JournalAmerican Journal of Clinical Pathology
Volume135
Issue number5
DOIs
Publication statusPublished - 1 May 2011

Fingerprint

Follicular Lymphoma
Lymphoma
B-Lymphocytes
Bone Marrow
Follicular Dendritic Cells
Germinal Center
Down-Regulation
Staining and Labeling

Keywords

  • Bone marrow
  • Follicular lymphoma
  • Germinal center
  • Immunohistochemistry
  • Low-grade B-cell lymphoma
  • Marginal zone lymphoma

Cite this

Younes, Sheren F. ; Beck, Andrew H. ; Ohgami, Robert S. ; Lossos, Izidore S. ; Levy, Ronald ; Warnke, Roger A. ; Natkunam, Yasodha. / The efficacy of HGAL and LMO2 in the separation of lymphomas derived from small B cells in nodal and extranodal sites, including the bone marrow. In: American Journal of Clinical Pathology. 2011 ; Vol. 135, No. 5. pp. 697-708.
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The efficacy of HGAL and LMO2 in the separation of lymphomas derived from small B cells in nodal and extranodal sites, including the bone marrow. / Younes, Sheren F.; Beck, Andrew H.; Ohgami, Robert S.; Lossos, Izidore S.; Levy, Ronald; Warnke, Roger A.; Natkunam, Yasodha.

In: American Journal of Clinical Pathology, Vol. 135, No. 5, 01.05.2011, p. 697-708.

Research output: Contribution to journalArticleResearchpeer-review

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